PrimerLab
Designing primers, not just writing code.
A lightweight, open-source toolkit for primer design, genomic analysis, and quality control — built for real research workflows.
Repetitive steps,
scattered tools.
Primer design is a critical but repetitive process in molecular biology. It often involves multiple separate steps — from sequence preparation and quality control to design, specificity checking, and evaluation. Existing tools are either too fragmented, command-line only, or lack a reproducible workflow for real research needs.
A unified, lightweight
toolkit.
PrimerLab brings essential primer design and genomic analysis steps into one CLI-based toolkit. It simplifies the workflow, integrates reliable bioinformatics tools, and makes the process more reproducible, efficient, and accessible for research and academic use.
Primer Design
Generate high-quality primers using Primer3.
Genomic QC
Check sequence quality and basic statistics.
Specificity Analysis
BLAST-based off-target checking.
SNP Awareness
Avoid primers overlapping known SNPs.
Multiplex Planning
Design compatible primer sets.
From sequence to validated primers.
Modular, simple,
and extensible.
Built with a modular structure, PrimerLab separates core functionalities into independent components, making it easy to maintain, extend, and integrate into larger workflows.
View architecture→From problem to tool.
I followed a simple process, from understanding the biological question, preparing the data, building the workflow, implementing the tool, validating the results, and documenting for future use.
Built with open tools.
- Core workflow
- Primer design
- Genomic QC
- Specificity analysis
- SNP awareness
- Multiplex planning
- Mintlify documentation
Better tools, better questions.
Through this project, I learned how to turn repetitive research steps into a clean, reproducible workflow, bridging biology and computation. It reminded me that good tools aren't just about code — they're about solving real problems for real people.
Explore the code.
PrimerLab is open-source and continuously improving. Contributions, feedback, and collaborations are welcome.
View on GitHub→